Coding

Part:BBa_K1365111:Design

Designed by: Rick Elbert   Group: iGEM14_Groningen   (2014-10-01)


dspB with signal sequence of USP45


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1048
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal restriction site was present in the signal sequence of USP45, therefore one nucleotide had to be changed resulting in a nonsense mutation and deletion of the PstI restriction site.


Source

ssUSP45DspB was isolated from the gblock synthesized by IDT.

References